| Description | BODIPY FL-X,SE, a green fluorescent dye designed for amine labeling, exhibits a high fluorescence quantum yield with minimal sensitivity to pH variations. It serves as a viable alternative to FAM, Cy2, or FITC. The dye's absorption and emission wavelengths are 504 nm (λ abs) and 510 nm (λ em), respectively. |
| In vitro | BODIPY FL-X, succinimidyl ester dye stock solutions are prepared in DMSO. Then, it is diluted in 0.1 M sodium bicarbonate buffer, pH 8.3, to a final concentration of 10 μM dye. |
| molecular weight | 502.33 |
| Molecular formula | C24H29BF2N4O5 |
| CAS | 217190-09-5 |
| Storage | keep away from direct sunlight | Powder: -20°C for 3 years | In solvent: -80°C for 1 year |
| References | 1. K P Top, et al.Green/red dual fluorescence detection of total protein and alkaline phosphate-conjugated probes on blotting membranes. Electrophoresis. 2001 Mar;22(5):896-905. 2. Karen Martin, et al. Simultaneous red/green dual fluorescence detection on electroblots using BODIPY TR-X succinimidyl ester and ELF 39 phosphate.Proteomics. 2002 May;2(5):499-512. |