| Description | ATP-Red 1 is a multisite-binding switchable fluorescent probe. It can rapidly and selectively respond to intracellular concentrations of ATP in living cells. |
| In vitro | The maximum absorption and emission wavelengths of ATP-Red 1 are 570/566 nm and 590/585 nm. ATP-Red 1 has good membrane permeability, and in the presence of 5?mM ATP, the fluorescence intensity of ATP-Red?1 increases 5.6-fold. ATP-Red?1 (2.5?μM, 20?min) shows much weaker fluorescence after KCN-induced inhibition of OXPHOS, which results in reduced mitochondrial ATP levels in OSCC cells. |
| molecular weight | 561.48 |
| Molecular formula | C34H36BN3O4 |
| CAS | 1847485-97-5 |
| Storage | keep away from direct sunlight | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice. |
| Solubility | DMSO: 50 mg/mL (89.05 mM), Sonication is recommended. |
| References | 1. Wang L, et al. A Multisite-Binding Switchable Fluorescent Probe for Monitoring Mitochondrial ATP Level Fluctuation in Live Cells. Angew Chem Int Ed Engl. 2016 Jan 26;55(5):1773-6 |