| Description | Aftin-4, an amyloid forty-two inducer, activates γ-secretase, promoting the generation of amyloid-β-1-42 (Aβ1-42) from amyloid-β protein precursor. Aftin-4(Aftin4) increased Aβ-1-42, but not Aβ-1-40 in mouse hipppocampus, accompanied by learning deficits and mitochondrial ultrastructural changes similar to those found in the brains of patients with AD. Aftin-4(Aftin4) can thus be used to induce a rapid, acute Alzheimer's disease-like toxicity in the rodent brain. |
| Animal experiments | Brains of 4-mo-old C57BL/6 wild-type mice were dissected and mechanically disassociated in HBSS with a tissue homogenizer. Aliquots from each mouse were incubated with DMSO and two concentrations of Aftin-4 at 37°C for 3 h. Liquefied brains were sonicated and centrifuged at 13,000 rpm for 15 min. WB analysis was performed using CT695 antibody for soluble Aβ-CTF. They are for reference only. |
| Synonyms | Aftin 4, Aftin4 |
| molecular weight | 368.48 |
| Molecular formula | C20H28N6O |
| CAS | 866893-90-5 |
| Storage | Powder: -20°C for 3 years | In solvent: -80°C for 1 year |
| Solubility | DMSO: 55 mg/mL (149.26 mM) |
| References | 1. Bettayeb K, etal. Small-molecule inducers of Aβ-42 peptide production share a common mechanism of action.[J]. Faseb Journal Official Publication of the Federation of American Societies for Experimental Biology, 2012, 26(12):5115-23. 2. Meunier J, etal. Brain toxicity and inflammation induced in vivo in mice by the amyloid-β forty-two inducer aftin-4, a roscovitine derivative[J]. Journal of Alzheimers Disease Jad, 2015, 44(2):507-24. 3. Hochard A, etal. Aftins increase amyloid-β42, lower amyloid-β38, and do not alter amyloid-β40 extracellular production in vitro: toward a chemical model of Alzheimer's disease [J]. Journal of Alzheimers Disease Jad, 2013, 35(1):107-120. |