| Description | ADHP (10-Acetyl-3,7-dihydroxyphenoxazine) is a fluorogenic peroxidase substrate (λex=530 nm, λem=590 nm). |
| In vitro | 为了获得Compound I的Km和kcat参数，采用了两种独立方法。首先，通过内置于荧光平板阅读器的注射器功能研究ADHP的氧化反应。自动注射器注入H2O2以触发反应，生成一系列进展曲线。对MPO介导的ADHP氧化分析显示，Km为31±4 μM，kcat为186±6/s。实验中ADHP浓度范围从1到80 μM，观测速率常数kobs随之增加；在将底物浓度固定在3到45 nM MPO的对照实验中亦是如此。从kobs对ADHP浓度作图的斜率得到的表观二级速率常数Kappon为2.1±0.2 mM/s。 |
| Synonyms | 10-乙酰基-3,7-二羟基吩嗪, 10-Acetyl-3,7-dihydroxyphenoxazine |
| molecular weight | 257.24 |
| Molecular formula | C14H11NO4 |
| CAS | 119171-73-2 |
| Storage | store at low temperature,store under nitrogen,keep away from direct sunlight | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice. |
| Solubility | DMSO: 60 mg/ml (233.25 mM) |
| References | 1. Jiansheng Huang, et al. Ordered Cleavage of Myeloperoxidase Ester Bonds Releases Active site Heme Leading to Inactivation of Myeloperoxidase by Benzoic Acid Hydrazide Analogs. Arch Biochem Biophys. 2014 Apr 15; 548: 74–85. |