| Description | Ac-DEVD-AFC is a fluorogenic substrate |
| In vitro | 通过荧光化合物N-acetyl-asp-glu-val-asp-7-amino-4-trifluoromethyl coumarin (Ac-DEVD-AFC)测量半胱天冬酶活性。研究表明，该底物快速进入细胞，在特定半胱天冬酶作用下有效地在天冬酰氨酸残基处被切割，产生荧光化合物7-amino-4-trifluoromethyl coumarin (AFC)。细胞破裂后，通过HPLC分离释放的AFC，并通过荧光检测。使用泛半胱天冬酶抑制剂benzyloxycarbonyl-val-ala-asp-fluoromethylketone可以阻断AFC在细胞中的出现，从而证实了细胞内半胱天冬酶负责该切割反应。在与多柔比星或放线霉素孵育约2小时后，首次观察到半胱天冬酶活性，之后AFC的产生随时间线性增长[1]。 |
| molecular weight | 729.61 |
| Molecular formula | C30H34F3N5O13 |
| CAS | 201608-14-2 |
| Storage | keep away from moisture | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice. |
| Solubility | DMSO: 40 mg/mL (54.82 mM) H2O: insoluble |
| References | 1. Tao Z , Goodisman J , Penefsky H S , et al. Caspase activation by anticancer drugs: the caspase storm.[J]. Molecular Pharmaceutics, 2007, 4(4):583. |