| Description | 740 Y-P acetate (740YPDGFR acetate) is a potent and cell-permeable PI3K activator.740 Y-P acetate tends to bind to GST fusion proteins containing the N- and C-terminal SH2 structural domains of p85, but not to GST alone. |
| In vitro | 740 Y-P TFA (1 μg/mL) 在测试的最低浓度下促进了有丝分裂。该肽在有无血清(0.5%)的条件下均可刺激有丝分裂，前者情况下，观察到最大反应浓度为50 μg/mL。740Y-P 对有丝分裂的刺激具有高度特异性，这一点与细胞渗透性SH2结合肽的一般特性不同。[1] 当740 Y-P TFA (50 μg/mL; 48小时)加入培养基时，其对有丝分裂的特异性刺激优于EGF或FGF刺激S期进入，显示在C2细胞中S期细胞的百分比为48.3%。此外，LY294002或wortmannin有效抑制了由740Y-P TFA肽刺激的有丝分裂反应。[1] 740 Y-P TFA (30 μM; 24小时) 显著抑制了GO诱导的PC12细胞中LC3-II/LC3-I的水平。[2] |
| Target activity | PI3K:20 μM |
| Synonyms | PDGFR 740Y-P Acetate, 740YPDGFR acetate, 740 Y-P acetate(1236188-16-1 Free base) |
| molecular weight | 3330.79 |
| Molecular formula | C143H226N43O41PS3 |
| Storage | store at low temperature,keep away from moisture|Powder: -20°C for 3 years | In solvent: -80°C for 1 year |
| Solubility | H2O: 83.3 mg/mL (25 mM) DMSO: 83.3 mg/mL (25 mM) |
| References | 1. Derossi D, et al. Stimulation of mitogenesis by a cell-permeable PI 3-kinase binding peptide. Biochem Biophys Res Commun. 1998;251(1):148-152. 2. Feng X, et al. Graphene oxide induces p62/SQSTM-dependent apoptosis through the impairment of autophagic flux and lysosomal dysfunction in PC12 cells. Acta Biomater. 2018;81:278-29 |