| Description | 6RK73 is a covalent irreversible and specific UCHL1 inhibitor (IC50: 0.23 µM). 6RK73 shows almost no inhibition of UCHL3 (IC50: 236 µM). |
| In vitro | 6RK73（5 µM；1-3小时）处理在MDA-MB-436细胞中显著抑制了TGFβ诱导的pSMAD2和pSMAD3，减少TβRI和总SMAD蛋白水平。6RK73（5 µM；24-48小时）在MDA-MB-436细胞中的迁移速率明显慢于DMSO对照组[1]。 |
| In vivo | 6RK73在斑马鱼体内显示出对乳腺癌转移的强效抑制作用[1]。 |
| Cell experiments | Cell Line: MDA-MB-436 cellsConcentration: 5 µMIncubation Time: 24, 48 hours |
| Target activity | UCHL1:0.23 µM , UCHL3:236 µM |
| molecular weight | 307.37 |
| Molecular formula | C13H17N5O2S |
| CAS | 1895050-66-4 |
| Storage | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice. |
| Solubility | DMSO: 112.5 mg/mL (366 mM), Sonication is recommended. |
| References | 1. Liu S, et al. Deubiquitinase activity profiling identifies UCHL1 as a candidate oncoprotein that promotes TGFβ-induced breast cancer metastasis. Clin Cancer Res. 2019 Dec 19. pii: clincanres.1373.2019. |