Description | 6-NBDG, a fluorescent glucose analogue, can be used as a fluorescent probe.6-NBDG can be used for monitoring glucose transport and uptake. 6-NBDG can be used as for detecting macrophage-rich atherosclerotic plaques. |
In vitro | 指南(本协议仅提供一个指导方针,根据您的具体需求进行相应修改)。将样本与不含葡萄糖的RPMI混合,其中含有200 μM 6-NBDG的最终浓度。使用弯曲镊子将样品嵌入混合物中,并通过放置在预热(40°C)玻璃片上的琼脂糖(40°C,20 μL)滴中拉伸样品。轻轻地将另一个预热的玻璃片放在样品上方,使得整个样品嵌入到琼脂糖-6-NBDG混合物中。使用共聚焦激光扫描显微镜拍摄共聚焦图像(Ex:471 nm,Em:500–600 nm)[2]。 |
molecular weight | 342.26 |
Molecular formula | C12H14N4O8 |
CAS | 108708-22-1 |
Storage | store at low temperature,keep away from direct sunlight|Powder: -20°C for 3 years | In solvent: -80°C for 1 year |
References | 1. Hansen TV, et al. Glucose Absorption by the Bacillary Band of Trichuris muris. PLoS Negl Trop Dis. 2016 Sep 2;10(9):e0004971. 2. Zaman RT, et al. Fiber-optic system for dual-modality imaging of glucose probes 18F-FDG and 6-NBDG in atherosclerotic plaques. PLoS One. 2014 Sep 18;9(9):e108108. |