| Description | 5-Chloro-2'-deoxyuridine (5-Chlorodeoxyuridine) is a thymine analog. It can be used to study the potential of hypochlorous acid damage to DNA and DNA precursors. |
| In vitro | The mammalian cells incorporate the analog into DNA when 5-Chloro-2’-deoxyuridine (ClDU) is placed into the tissue culture medium. It is observed that a 10 μM concentration of 5-Chloro-2’-deoxyuridine in the media does not alter cell division kinetics. Previously it has been shown that 5-Chloro-2’-deoxyuridine is metabolized and incorporated into DNA using antibodies that bind selectively to DNA containing halogenated bases. In this study, 5-Chloro-2’-deoxyuridine is more similar to BrdU in acting as a T analog. The toxicity of 5-Chloro-2’-deoxyuridine could in part be attributed to inhibition of thymidylate synthase [1]. |
| Synonyms | 5-Chlorodeoxyuridine, 5-氯-2-脱氧尿嘧啶核苷, CldU |
| molecular weight | 262.65 |
| Molecular formula | C9H11ClN2O5 |
| CAS | 50-90-8 |
| Storage | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice. |
| Solubility | DMSO: 125 mg/mL (475.92 mM) |
| References | 1. Kim CH, et al. Polymerase incorporation and miscoding properties of 5-chlorouracil. Chem Res Toxicol. 2010 Apr 19;23(4):740-8. 2. Yuan CJ, et al. Extended access methamphetamine decreases immature neurons in the hippocampus which results from loss and altered development of neural progenitors without altered dynamics of the S-phase of the cell cycle. Pharmacol Biochem Behav. 2011 Nov;100(1):98-108. |