| Description | The cell-permeant pH indicator, carboxy SNARF-1, acetoxymethyl ester, acetate (CAS #126208-13-7) has a pKa of ~7.5 after de-esterification, thus is useful for measuring pH changes between pH 7 and pH 8. Carboxy-SNARF-1 exhibits a significant pH-dependent emission shift from yellow-orange to deep red fluorescence under acidic and basic conditions, respectively. This pH dependence allows the ratio of the fluorescence intensities from the dye at two emission wavelengths – typically 580 nm and 640 nm – to be used for quantitative determinations of pH. |
| In vitro | Incubating cells in 1～10 μM SNARF AM acetate for 30 minutes at the optimum temperature for the specific cell type of interest is recommend. Then, cells should be washed before pH measurements. |
| molecular weight | 567.55 |
| Molecular formula | C32H25NO9 |
| CAS | 126208-13-7 |
| Storage | keep away from direct sunlight | Powder: -20°C for 3 years | In solvent: -80°C for 1 year |
5-(and-6)-Carboxy SNARF-1, Acetoxymethyl Ester, Acetate
CAS No.: 126208-13-7
The cell-permeant pH indicator, carboxy SNARF-1, acetoxymethyl ester, acetate (CAS #126208-13-7) has a pKa of ~7.5 after
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