| Description | 5(6)-FITC (Fluorescein isothiocyanate), or Fluorescein 5(6)-isothiocyanate, is a versatile amine-reactive derivative of fluorescein dye. It serves as an effective label for antibodies and other probes, finding utility in fluorescence microscopy, flow cytometry, and immunofluorescence-based assays such as ELISA and Western blotting. |
| Synonyms | Fluorescein 5(6)-isothiocyanate, 异硫氰酸荧光素, Fluorescein isothiocyanate |
| molecular weight | 389.38 |
| Molecular formula | C21H11NO5S |
| CAS | 27072-45-3 |
| Storage | keep away from direct sunlight | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice. |
| Solubility | DMSO: 160 mg/mL (410.91 mM) |
| References | 1. Goding JW. Conjugation of antibodies with fluorochromes:modifications to the standard methods. J Immunol Methods. 1976;13(3-4):215-26. 2. Der-Balian GP, et al. Fluorescein labeling of Fab' while preserving single thiol. Anal Biochem. 1988 Aug 15;173(1):59-63. 3. Smith LM, et al. Fluorescence detection in automated DNA sequence analysis. Nature. 1986 Jun 12-18;321(6071):674-9. |
| Citations | 1. Zhang J, Zhou E C, He Y, et al.ZYG11B potentiates the antiviral innate immune response by enhancing cGAS-DNA binding and condensation.Cell Reports.2023, 42(3). |