4-Nitrophenyl α-D-galactopyranoside Bioactivity,Chemical Properties,Storage & Solubility Information Pathways-PeptideDB

Description	4-Nitrophenyl α-D-galactopyranoside (PNP-alpha-D-Gal) (PNP-alpha-D-Gal) is an artificial substrate of 4-nitrophenyl (pNP) glycopyranoside for detecting α-galactosidase activity. The amount of released pNP is significantly increased when PNP-alpha-D-Gal is used as substrates.
In vitro	Using 4-nitrophenol (pNP)-glycopyranosides as pseudosubstrates, the amount of liberated pNP from the 4-Nitrophenyl α-D-galactopyranoside is significantly higher than that from the other pNP-glycopyranosides at pH 4.0 while no activity was detected at pH 7.0. The digestion assays using pNP glycopyranosides as artificial substrates exhibit that yielding liberated galactose residues from 4-Nitrophenyl α-D-galactopyranoside mainly at pH 4.0, i.e. the pH level where yield threshold is decreased at most.
Synonyms	PNP-alpha-D-Gal, 4-硝基苯-α-D-吡喃半乳糖苷, PNP-α-D-Gal
molecular weight	301.25
Molecular formula	C12H15NO8
CAS	7493-95-0
Storage	Powder: -20°C for 3 years \| In solvent: -80°C for 1 year \| Shipping with blue ice.
Solubility	DMSO: 50 mg/mL (165.98 mM)
References	1. Okamoto-Nakazato A. Implications of the galactosidase activity of yieldin in the regulatory mechanism of yield threshold that is fundamental to cell wall extension. Physiol Plant. 2018 Jun;163(2):259-266.

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