| Description | 4-Methylumbelliferyl oleate is a fluorescent substrate for lipases (Ex/Em=320/450 nm), which can be cleaved by lipase and can be used to measure pancreatic inhibitory activity. |
| In vitro | 该化合物对胰脂肪酶的抑制活性是通过使用4-Methylumbelliferyl (4-MU) oleate作为底物来衡量的。反应混合物包括50 µL 0.1 mM的4-MU oleate，20 µL的McIlvane缓冲液（0.1 M柠檬酸-Na2HPO4，pH 7.4）以及5 µL的样品溶液。随后加入25 µL的猪胰脂肪酶至反应混合物中，并将总体积调整至0.1 ml。混合物在37℃下孵育10分钟后，通过在激发波长320 nm和发射波长450 nm下使用荧光多功能检测仪来测量脂肪酶释放的4-MU的量[1]。 |
| molecular weight | 440.62 |
| Molecular formula | C28H40O4 |
| CAS | 18323-58-5 |
| Storage | keep away from moisture | store at -20°C | Shipping with blue ice. |
| Solubility | DMSO: 80 mg/mL (181.57 mM), Sonication is recommended. |
| References | 1. Zhang J, et al. Pancreatic lipase inhibitory activity of taraxacum officinale in vitro and in vivo. Nutr Res Pract. 2008 Winter;2(4):200-3. 2. Koster JF, et al. Study of the hydrolysis of 4-methylumbelliferyl oleate by acid lipase and cholesteryl oleate by acid cholesteryl esterase in human leucocytes, fibroblasts and liver. Biochim Biophys Acta. 1980 Apr 18;618(1):98-105. |