| Description | 4',5'-Dibromofluorescein (Eosinic acid) is a fluorescent dye that can be used as a ligand for protein characterization by spectroscopic analysis. |
| Synonyms | CCRIS 4900, C.I. Solvent Red 72, Solvent Red 72, C Orange no. 5, Japan Orange No. 201, Eosinic acid, D & |
| molecular weight | 490.1 |
| Molecular formula | C20H10Br2O5 |
| CAS | 596-03-2 |
| Storage | keep away from direct sunlight | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice. |
| Solubility | DMSO: 56.2 mg/mL (114.8 mM), Sonication is recommended. |
| References | 1. Yang HH, et al. Determination of synthetic by-products and an intermediate in the colour additive D&C Orange No. 5 using high-performance liquid chromatography. Food Addit Contam Part A Chem Anal Control Expo Risk Assess. 2012;29(9):1386-1390. 2. Fu S, et al. Study on the resonance Rayleigh scattering spectra of the interactions of palladium (II)-cephalosporins chelates with 4,5-dibromofluorescein and their analytical applications. Anal Chim Acta. 2007;599(2):271-278. 3. Yu D, et al. An ultrasensitive stain for negative protein detection in SDS-PAGE via 4',5'-Dibromofluorescein. J Proteomics. 2017;165:21-25. 4. Ritchie EE, et al. Ecotoxicity of xanthene dyes and a non-chlorinated bisphenol in soil. Chemosphere. 2013;90(7):2129-2135. |