| Description | The DYKDDDDK-tag system utilizes a short, hydrophilic 8- amino-acid peptide that is fused to the protein of interest1. The DYKDDDDK peptide binds to the antibody M1. To improve the detection of the DYKDDDDK tag the 3x (DYKDDDDK) system has been developed. This three tandem DYKDDDDK epitope is hydrophilic, 22-amino-acids long, and can detect up to 10 fmol of the expressed fusion protein. The DYKDDDDK-tagged maltodextrin-binding protein of Pyrococcus furiosus has been crystallized4 and the quality of the crystals was very similar to that of crystals of untagged protein. |
| molecular weight | 2861.87 |
| Molecular formula | C120H169N31O49S |
| Storage | keep away from moisture | Powder: -20°C for 3 years | In solvent: -80°C for 1 year |
| Solubility | TBS (0.5M Tris-HCl, pH 7.4, with 1M NaCl): ≥25 mg/mL |