| Description | 3,5-Dinitrocatechol is an inhibitor of catechol-O-methyl-transferase(COMT) and can be used to prepare the molybdenum (VI)- 3,5-Dinitrocatechol complex. |
| In vitro | 3,5-Dinitrocatechol is highly effective (IC50 = 12 nM) and selective in inhibiting COMT activity in vitro[3]. |
| In vivo | In mouse trachea (β1-AR dominant), significant suppression of ISO-induced relaxation was observed with the addition of 3,5-Dinitrocatechol . In GP trachea, in the presence of clorgiline plus 3,5-Dinitrocatechol , both normetadrenaline (NMA) and metadrenaline (MA) (10-4 M) significantly suppressed ISO-induced relaxation[1]. |
| Target activity | COMT:12 nM |
| molecular weight | 200.11 |
| Molecular formula | C6H4N2O6 |
| CAS | 7659-29-2 |
| Storage | Powder: -20°C for 3 years | In solvent: -80°C for 1 year |
| Solubility | Ethanol: < 20.01 mg/mL DMSO: 50 mg/mL (249.86 mM) H2O: 0.17 mg/mL |
| References | 1. Yamaki F, et, al. Effects of Catecholamine Metabolites on Beta-Adrenoceptor-Mediated Relaxation of Smooth Muscle: Evaluation in Mouse and Guinea-Pig Trachea and Rat Aorta. Biol Pharm Bull. 2020;43(3):493-502. 2. Stojnova K, et, al. Study on the Complex Equilibria of Molybdenum(VI) with 3,5-Dinitrocatechol and Ditetrazolium Salt. Acta Chim Slov. 2016;63(3):654-60. 3. Nissinen E, et, al. Inhibition of catechol-O-methyltransferase activity by two novel disubstituted catechols in the rat. Eur J Pharmacol. 1988 Aug 24;153(2-3):263-9. |