| Description | 2-Bromohexadecanoic acid (2-BP) is a non-metabolizable palmitate analog, an agonist of PPARδ, which acts as a palmitoylation inhibitor and inhibits DHHC-mediated palmitoylation. |
| In vitro | 方法: HepG2 用 PA (200 µM) 和 2-Bromohexadecanoic acid (25-150 µM) 处理，检测 CSC 球体形态和数量。结果: 2-Bromohexadecanoic acid (25、50 和 150 µM) 显著降低了PA诱导的HepG2 CSC球体的形成 (75-150 µM 和 >150 µM。[1]方法: ERα 或 ERα-Cys447Ala 转染的 HeLa 细胞和 HepG2 细胞用 2-Bromohexadecanoic acid (10 µM) 预处理 30 min，再加 E2 (10 nM) 刺激 10 min，使用 Western Blot 检测靶点蛋白表达水平。结果: E2 在 ERα 转染的 HeLa 细胞和 HepG2 细胞中诱导 ERK 和 AKT 磷酸化。如果用 PAT 抑制剂 2-Bromohexadecanoic acid 预处理细胞，E2 诱导的信号激酶激活被完全阻断，而不影响磷酸化的基础水平。E2 在诱导 ERα-Cys447Ala 突变体转染的 HeLa 细胞中 ERK 和 AKT 磷酸化方面是无效的。[2] |
| Target activity | Palmitoylation: |
| Synonyms | 2-BP, 2-溴十六烷酸, 2-bromopalmitate, 2-Bromohexadecanoic acid |
| molecular weight | 335.32 |
| Molecular formula | C16H31BrO2 |
| CAS | 18263-25-7 |
| Storage | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice. |
| Solubility | Methanol: 100 mg/mL DMSO: 60 mg/mL (178.93 mM) |
| References | 1. Chong LW, et al. Targeting protein palmitoylation decreases palmitate‑induced sphere formation of human liver cancer cells. Mol Med Rep. 2020 Aug;22(2):939-947. 2. Acconcia F, et al. Palmitoylation-dependent estrogen receptor alpha membrane localization: regulation by 17beta-estradiol. Mol Biol Cell. 2005 Jan;16(1):231-7. |