| Description | Vanillin (Zimco) is a single molecule extracted from vanilla beans and also a popular odor used widely in perfume, food and medicine.Vanillin can reversibly and non-competitively inhibit the cellulase activity at appropriate concentrations and the value of IC50 was estimated to be 30 g/L.Vanillin protects KSC from UVB irradiation and its effects may occur through the suppression of downstream step of MDM2 in UVB irradiation-induced p53 activation. Vanillin also inhibits yeast growth and fermentation. |
| In vivo | Vanillin 的LD50（口服）分别对大鼠、豚鼠和兔子为1.58克/千克、1.40克/千克、3.0克/千克。多项针对大鼠的口服毒性研究表明，多周期摄入Vanillin 未见副作用。 |
| Synonyms | Lioxin, m-Methoxy-p-hydroxybenzaldehyde, 香草醛, Zimco, p-Vanillin, p-Hydroxy-m-methoxybenzaldehyde, 香兰素, FEMA 3107 |
| molecular weight | 152.15 |
| Molecular formula | C8H8O3 |
| CAS | 121-33-5 |
| Storage | Powder: -20°C for 3 years | In solvent: -80°C for 1 year |
| Solubility | Ethanol: 28 mg/mL (184 mM) DMSO: 50 mg/mL (328.62 mM) H2O: < 1 mg/mL (insoluble or slightly soluble) |
| References | 1. Abraham DJ, et al. Blood, 1991, 77(6), 1334-1341. 2. Shim K S, Hwang Y H, Jang S A, et al. Ethanol Extract of Amomum tsao-ko Ameliorates Ovariectomy-Induced Trabecular Loss and Fat Accumulation[J]. Molecules. 2021, 26(4): 784. |
| Kinase | Ligand Binding Assay: To determine the binding affinity of T0070907 to the PPARs, scintillation proximity assay (SPA) is performed with the following modifications. A 90-μl reaction contains SPA buffer (10 mm KH2PO4, 10 mm KH2PO4, 2 mm EDTA, 50 mm NaCl, 1 mm dithiothreitol, 2 mmCHAPS, 10% (v/v) glycerol, pH 7.1), 50 ng of GST-PPARγ (or 150 ng of GST-PPARα, GST-PPARδ), 5 nm 3H-labeled radioligands, and 5 μl of T0070907 in Me2SO. After incubation for 1 h at room temperature, 10 μl of polylysine-coated SPA beads (at 20 mg/ml in SPA buffer) are added, and the mixtureis incubated for 1 h before reading in Packard Topcount. [3H]Rosiglitazone is used for PPARγ, and [3H]GW2433 is used for PPARα and PPARδ. |