| Description | Swinholide A is a polyketone compound that can be extracted from sponges and binds to actin to dimerize actin with Kd of about 50 nM. Swinholide A is a cytotoxin that stabilizes actin dimers and severs actin filaments. Swinholide A has antifungal activity and can damage the actin cytoskeleton of cells. |
| In vitro | First isolated from the Okinawan marine sponge Theonella swinhoei, Swinholide A dimerizes actin[1].Isolated from the marine sponge Theonella swinhoei, Swinholide A is highly cytotoxic to a variety of cancer cell lines[2].Disrupting the actin cytoskeleton of cells grown in culture, Swinholide A sequesters actin dimers in vitro in both polymerizing and non-polymerizing buffers, with a binding stoichiometry of one Swinholide A molecule per actin dimer. Additionally, it rapidly severs F-actin in vitro with high cooperativity[2]. |
| Synonyms | MK-7684 |
| molecular weight | 1389.87 |
| Molecular formula | C78H132O20 |
| CAS | 95927-67-6 |
| Storage | store at low temperature | Powder: -20°C for 3 years | In solvent: -80°C for 1 year |
| Solubility | Methanol: Soluble DMSO: Soluble Ethanol: Soluble |
| References | 1. Inji Shin,et al. Total Synthesis of Swinholide A: An Exposition in Hydrogen-Mediated C-C Bond Formation. J Am Chem Soc. 2016 Nov 2;138(43):14246-14249. 2. M R Bubb, et al. Swinholide A is a microfilament disrupting marine toxin that stabilizes actin dimers and severs actin filaments. J Biol Chem. 1995 Feb 24;270(8):3463-6. 3. Terry, D.R., Higa, T., and Bubb, M.R. Misakinolide A is a marine macrolide that caps but does not sever filamentous actin. The Journal of Biological Chemisty 272(12), 7841-7845 (1997) |