| Description | Stachydrine (N,N-Dimethyl-L-proline) is a major constituent of Chinese herb leonurus heterophyllus sweet used to promote blood circulation and dispel blood stasis. Stachydrine (N,N-Dimethyl-L-proline) can inhibit the NF-κB signal pathway. Stachydrine (N,N-Dimethyl-L-proline) is a biomarker for the consumption of citrus fruits. |
| In vitro | Various concentrations of stachydrine intervention (10(-6) - 10(-4) mol/L) showed better anti-hypertrophic effect and decreased the number of ROS positive myocardial cell (P<0.05). Meanwhile, intervention of stachydrine significantly suppressed the level of p-IkappaB (ser32) protein in the cytosol and NF-kappaB (p65) protein in the nucleus (P<0.05). |
| Cell experiments | Myocardial cells of neonatal rats were incubated with angiotensin II for 48 hours to establish myocardial cell hypertrophy models. The cell surface area and protein/DNA ratio were detected as index for hypertrophy of myocardial cells. ROS positive myocardial cell were counted by means of flow cytometry, the expression of p-IkappaB (ser32) protein in the cytosol and NF-kappaB (p65) protein in the nucleus were also detected by Western blot. |
| Synonyms | Methyl hygrate betaine, 水苏碱, L-stachydrine, Proline betaine, N,N-Dimethyl-L-proline |
| molecular weight | 143.18 |
| Molecular formula | C7H13NO2 |
| CAS | 471-87-4 |
| Storage | Powder: -20°C for 3 years | In solvent: -80°C for 1 year |
| Solubility | DMSO: 26 mg/mL (181.59 mM) H2O: 32 mg/mL (223.49 mM) |
| References | 1. Guo W , Zhang C , Liao Y L , et al. [Effect of Leonurus stachydrine on myocardial cell hypertrophy][J]. Zhong yao cai = Zhongyaocai = Journal of Chinese medicinal materials, 2012, 35(6):940-943. |