| Description | Rutin (Quercetin 3-O-rutinoside) belongs to the flavonoid group of natural products with blood-brain barrier permeability. Rutin has a wide range of biological activities including antioxidant, anti-inflammatory, anti-tumor, hypoglycemic, neuroprotective, anti-bacterial, anti-aging and so on. |
| In vitro | 方法：不同的肿瘤细胞系用 Rutin (5-20 µM) 处理 24 h，使用 MTT assay 测定细胞活力。结果：Rutin 以浓度依赖的方式抑制不同细胞系的活力。肿瘤细胞系 SH-SY5Y、Neuro-2a、CHME、U-118、LN-229 和 SK-N-SH 的 IC50 值分别为 >40、24±4.3、15±2.6、>40、22±4.8 和 36±3.7 µM。[1]方法：人类神经母细胞瘤细胞 LAN-5 用 Rutin (25-100 µM) 处理 24 h，使用 Annexin V-FITC/PI apoptosis assay 检测细胞凋亡情况。结果：不同剂量 Rutin 的加入诱导了 LAN-5 细胞的凋亡，凋亡细胞的百分比从 4.48% 增加到 7.50%、11.26% 和 20.37%。[2] |
| In vivo | 方法：为测试体内抗癫痫活性，将 Rutin (100-200 mg/kg) 腹腔注射给 BALB/c 小鼠，每天一次，持续七天。最后一天注射 kainic acid (10 mg/kg) 诱导癫痫。结果：Rutin 组癫痫发作评分明显低于对照组。Kainic acid 给药后第 1 天和第 21 天，Rutin 组海马丙二醛水平显著低于对照组。Rutin 组在 kainic acid 给药后第 1 天观察到的硫醇水平高于对照组。[3] |
| Synonyms | Quercetin 3-O-rutinoside, 芦丁, Rutoside |
| molecular weight | 610.52 |
| Molecular formula | C27H30O16 |
| CAS | 153-18-4 |
| Storage | store at low temperature,store under nitrogen | Powder: -20°C for 3 years | In solvent: -80°C for 1 year |
| Solubility | DMSO: 60 mg/mL (98.28 mM) Ethanol: 2 mg/mL (3.27 mM) |
| References | 1. Yan X, et al. Rutin induces apoptosis via P53 up-regulation in human glioma CHME cells. Transl Cancer Res. 2019 Sep;8(5):2005-2013. 2. Chen H, et al. Anti-tumor effect of rutin on human neuroblastoma cell lines through inducing G2/M cell cycle arrest and promoting apoptosis. ScientificWorldJournal. 2013 Dec 29;2013:269165. 3. Nassiri-Asl M, et al. Effects of rutin on oxidative stress in mice with kainic acid-induced seizure. J Integr Med. 2013 Sep;11(5):337-42. 4. Shim K S, Hwang Y H, Jang S A, et al. Ethanol Extract of Amomum tsao-ko Ameliorates Ovariectomy-Induced Trabecular Loss and Fat Accumulation[J]. Molecules. 2021, 26(4): 784. |