| Description | Resveratrol (SRT 501) is a polyphenolic natural product, a plant antitoxin with antioxidant and chemopreventive activities. Resveratrol has a wide range of targets including COX, SIRT, LOC, etc. Resveratrol induces autophagy and apoptosis. |
| In vitro | 方法：小鼠乳腺癌细胞 4T1 用 Resveratrol (50-250 μM) 处理 72 h，使用 CCK-8 检测细胞活力。结果：Resveratrol 剂量依赖性抑制 4T1 细胞的活力，IC50 为 93 μM。[1]方法：人乳腺癌细胞 MCF-7 用 Resveratrol (1-100 μM) 处理 24 h，使用 luciferin-luciferase assay 检测细胞内 ATP 含量。结果：Resveratrol 降低了细胞 ATP 含量。在 1 μM Resveratrol 时降低了约 16%，在 100 μM Resveratrol 时降低了 50%。[2] |
| In vivo | 方法：为检测体内抗肿瘤活性，将 Resveratrol (50-100 mg/kg，5% ethanol and 25% polyethyleneglycol 400 in distilled water) 腹腔注射给携带人卵巢畸胎瘤 PA-1 的 BALB/c (nu/nu) 小鼠，每天一次，持续四周。结果：Resveratrol 抑制 PA-1 细胞异种移植物的生长，抑制 eEF1A2 的表达。[3]方法：为研究对抑郁症小鼠的影响，将 Resveratrol (10-30 mg/kg) 腹腔注射给抑郁症模型的 C57BL/6 小鼠，每天一次，持续二十一天。结果：Resveratrol 可显著提高前额叶皮层神经递质 DA 和 5-HT 的水平，并增加大脑中 NPY 的表达，这对抑郁症起到拮抗作用。[4] |
| Cell experiments | HAECs were treated with RSV in the presence of isobutylmethylxanthine (IBMX). The reaction was stopped by adding ice-cold 6% trichloroacetic acid and the supernatant fractions of the cellular lysates were extracted, dried and acetylated. Cyclic GMP levels were determined by an enzyme immunoassay kit and results standardized by protein levels [5]. |
| Animal experiments | Eight-week male apoE-/- mice (C57BL/6 background) were used in the study. Mice were housed in photobiologic light-exposure chambers with a 12:12-h light:dark cycle, and eat food ad libitum. Mice were randomly divided into two groups: one group receiving a high cholesterol diet (HCD, 21% fat, 19.5% casein, and 1.5% cholesterol), the other receiving HCD supplemented with RSV (200 mg/100 g diet). All mice were fed for 8 weeks. To identify the potential role of PKA, some mice were treated with PKA specific inhibitor (KT5720, 1.2 mg/kg) via intraperitoneal injection once a day at the last 4 weeks. The animal experiments were conducted according to the institutional guidelines of Guangdong Provincial People's Hospital [5]. |
| Target activity | COX-1:1.1 μM (cell free), COX-2:1.3 μM (cell free), DNA polymerase α:3.3 μM (cell free), DNA polymerase δ:5 μM (cell free) |
| Synonyms | 白藜芦醇, trans-Resveratrol, SRT 501 |
| molecular weight | 228.24 |
| Molecular formula | C14H12O3 |
| CAS | 501-36-0 |
| Storage | Powder: -20°C for 3 years | In solvent: -80°C for 1 year |
| Solubility | DMSO: 60 mg/mL (262.88 mM) H2O: < 1 mg/mL (insoluble or slightly soluble) Ethanol: < 1 mg/mL (insoluble or slightly soluble) |
| References | 1. Wu H, et al. The Cytotoxicity Effect of Resveratrol: Cell Cycle Arrest and Induced Apoptosis of Breast Cancer 4T1 Cells. Toxins (Basel). 2019 Dec 13;11(12):731. 2. Gomez LS, et al. Resveratrol decreases breast cancer cell viability and glucose metabolism by inhibiting 6-phosphofructo-1-kinase. Biochimie. 2013 Jun;95(6):1336-43. 3. Lee MH, et al. Resveratrol suppresses growth of human ovarian cancer cells in culture and in a murine xenograft model: eukaryotic elongation factor 1A2 as a potential target. Cancer Res. 2009 Sep 15;69(18):7449-58. 4. Gu Z, et al. Therapeutic effect of resveratrol on mice with depression. Exp Ther Med. 2019 Apr;17(4):3061-3064. 5. Li J, et al. Resveratrol improves endothelial dysfunction and attenuates atherogenesis in apolipoprotein E-deficient mice. J Nutr Biochem. 2019 Feb 10;67:63-71. 6. Lu J, Lu Z, Liu L, et al. Identification of crocin as a new hIAPP amyloid inhibitor via a simple yet highly biospecific screening system[J]. Chemistry & Biodiversity. 2021 7. Pang K, Li B, Tang Z, et al. Resveratrol inhibits hypertrophic scars formation by activating autophagy via the miR-4654/Rheb axis[J]. Molecular medicine reports. 2020, 22(4): 3440-3452. 8. Zhang K, Pan X, Zheng J, et al. SIRT1 protects against aortic dissection by regulating AP-1/decorin signaling-mediated PDCD4 activation[J]. Molecular Biology Reports. 2020, 47(3): 2149-2159. 9. Zhou H, Ning Y, Zeng G, et al. Curcumin promotes cell cycle arrest and apoptosis of acute myeloid leukemia cells by inactivating AKT[J]. Oncology Reports. 2021, 45(4): 1-1. 10. Geng W, Guo X, Zhang L, et al. Resveratrol inhibits proliferation, migration and invasion of multiple myeloma cells via NEAT1-mediated Wnt/β-catenin signaling pathway[J]. Biomedicine & Pharmacotherapy. 2018 Nov;107:484-494. |