| Description | Punicalagin, a major ellagitannin found in pomegranate, is a reversible and non-competitive inhibitor of 3CLpro. Punicalagin exhibits antioxidant, anti-inflammatory, and antitumor activities. |
| In vitro | 方法：人宫颈细胞 ME-180 用 Punicalagin (10-100 µM) 处理 24 h，使用 MTT assay 检测细胞活力。结果：Puncalagin 对 ME-180 细胞具有显著的浓度依赖性细胞毒性。与对照细胞相比，Punicalagin 处理的细胞的细胞活力降低了约 80%。[1]方法：人宫颈癌细胞 HeLa 用 Punicalagin (25-100 µM) 处理 36 h，使用 Flow cytometry 检测细胞周期。结果：Punicalagin 处理 36 h 后，G1 期细胞数量显著增加。[2] |
| In vivo | 方法：为研究对 MTX 诱导的小鼠肝毒性的影响，将 Punicalagin (25-50 mg/kg, 0.5% CMC) 口服给药给 Swiss albino 小鼠，每天一次，持续十天，在第七天腹膜内注射单剂量 MTX (20 mg/kg)。结果：Punicalagin 显著减弱了 MTX 诱导的血清转氨酶、ALP 和 LDH 升高以及肝脏氧化应激措施，并增强了肝脏的抗氧化防御。在 MTX 诱导的小鼠肝脏中，Punicalagin 抑制氧化损伤、炎症和细胞凋亡，并上调 Nrf2。[3] |
| Synonyms | 安石榴甙, 安石榴苷 |
| molecular weight | 1084.72 |
| Molecular formula | C48H28O30 |
| CAS | 65995-63-3 |
| Storage | keep away from direct sunlight | Powder: -20°C for 3 years | In solvent: -80°C for 1 year |
| Solubility | DMSO: 55 mg/mL (50.7 mM) |
| References | 1. Zhang L, et al. Punicalagin promotes the apoptosis in human cervical cancer (ME-180) cells through mitochondrial pathway and by inhibiting the NF-kB signaling pathway. Saudi J Biol Sci. 2020 Apr;27(4):1100-1106. 2. Tang J, et al. Punicalagin suppresses the proliferation and invasion of cervical cancer cells through inhibition of the β-catenin pathway. Mol Med Rep. 2017 Aug;16(2):1439-1444. 3. Al-Khawalde AAA, et al. Punicalagin Protects against the Development of Methotrexate-Induced Hepatotoxicity in Mice via Activating Nrf2 Signaling and Decreasing Oxidative Stress, Inflammation, and Cell Death. Int J Mol Sci. 2022 Oct 15;23(20):12334. |