| Description | Puerarin 6''-O-xyloside possesses significant anti-osteoporotic activity on ovariectomy mice, it has significant antitumour activities, the mechanisms are related to increased levels of caspase-3, caspase-7, caspase-9 and Bax, and reduced levels Bcl-2. |
| In vivo | The in vivo animal study showed that POS significantly inhibited tumour growth in A549 cells (p? |
| Animal experiments | The 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay was used to determine the cytotoxicity of POS (at 10, 20 and 40?μM) in vitro, and xenograft nude mice were established to evaluate the antitumour effect of POS (at 40?mg/kg/d) in vivo by 15 days intraperitoneal injection (ip). To explore its mechanism of action, flow cytometry was performed to determine the pro-apoptotic effect of POS (at 10, 20 and 40?μM). Subsequently, the expression of caspase-3, caspase-7, caspase-9, Bcl-2 and Bax in A549 cells were determined[1]. |
| molecular weight | 548.49 |
| Molecular formula | C26H28O13 |
| CAS | 114240-18-5 |
| Storage | Powder: -20°C for 3 years | In solvent: -80°C for 1 year |
| Solubility | DMSO: 25 mg/mL (45.58 mM) |
| References | 1. Chen T , Chen H , Wang Y , et al. In vitro and in vivo antitumour activities of puerarin 6″-O-xyloside on human lung carcinoma A549 cell line via the induction of the mitochondria-mediated apoptosis pathway[J]. Pharmaceutical Biology. 2. Li H , Chen B , Pang G , et al. Anti-osteoporotic activity of puerarin 6″-O-xyloside on ovariectomized mice and its potential mechanism[J]. Pharmaceutical Biology, 2015, 54(1):1-7. |