| Description | PACAP (1-27), human, ovine, rat acetate (PACAP 1-27 acetate) is a novel neuropeptides originally isolated from bovine hypothalamus, also found in humans and rats. |
| In vitro | 通过对I-monoiodinated PACAP (1-27), human, ovine, rat进行放射配体受体结合实验，确认AR4-2J细胞表面存在PAC受体，因为PACAP (1-27), human, ovine, rat和PACAP(1–38)以相同的效力（Kd值为1-2 nM）置换放射配体的结合，而血管活性肠肽(VIP)的效力则低1000倍。PACAP (1-27), human, ovine, rat对VIP-氨基酸替换表现出明显更高的敏感性。在AR4-2J细胞中，PACAP (1-27), human, ovine, rat展现出促进IP3和cAMP生成的效能和结合亲和力。 |
| In vivo | 垂体腺苷酸环化酶激活多肽(PACAP (1-27), human, ovine, rat)对麻醉、通气的豚鼠总肺阻力(RL)增加的抑制作用进行了研究，该增加由过敏原或组胺引起。通过静脉输注给予PACAP (1-27), human, ovine, rat（剂量范围0.045-4.5 nmol/kg/min）能剂量依赖性地减少吸入卵清蛋白和组胺引起的RL增加。在最高剂量时，PACAP (1-27), human, ovine, rat完全阻止了由卵清蛋白和组胺引起的RL增加。PACAP (1-27), human, ovine, rat与β2-肾上腺素受体激动剂，沙丁胺醇（剂量范围0.045-4.5 nmol/kg/min）的输注同样抑制RL增加，但沙丁胺醇比PACAP (1-27), human, ovine, rat更能增加心率[3]。 |
| Synonyms | PACAP 1-27 acetate, PACAP (1-27), human, ovine, rat acetate(127317-03-7 free base) |
| molecular weight | 3207.71 |
| Molecular formula | C144H228N40O41S |
| Storage | keep away from moisture | Powder: -20°C for 3 years | In solvent: -80°C for 1 year |
| Solubility | DMSO: 5 mM |
| References | 1. Gourlet P, et al. Fragments of pituitary adenylate cyclase activating polypeptide discriminate between type I and II recombinant receptors. Eur J Pharmacol. 1995 Dec 4;287(1):7-11. |