| Description | N2-methylguanine (N2-methylguanine) is an endogenous methylated nucleoside found in human fluids and a modified nucleoside. |
| In vitro | N2-methylguanine 是位于大肠杆菌16S rRNA 5'端起始1340个碱基附近，对反转录的主要动力学障碍。转录中间体对应于rRNA序列中N2-methylguanine 位置的衰减。通过m2G延伸cDNA的松弛时间约为3分钟[1]。N2-methylguanine 存在于RNA二级结构的螺旋和环状区域，且可呈现s-cis或s-trans转换体。若甲基团存在旋转偏好，则N2-methylguanine 的取代效应可能具序列环境特异性，这取决于哪一个碱基面参与氢键形成[2]。 |
| Synonyms | 6-羟基-2-氨甲基嘌呤 |
| molecular weight | 165.15 |
| Molecular formula | C6H7N5O |
| CAS | 10030-78-1 |
| Storage | Powder: -20°C for 3 years | In solvent: -80°C for 1 year |
| Solubility | DMSO: < 1 mg/mL (insoluble or slightly soluble) |
| References | 1. Youvan DC, et al. Reverse transcriptase pauses at N2-methylguanine during in vitro transcription of Escherichia coli 16S ribosomal RNA. Proc Natl Acad Sci U S A. 1979 Aug;76(8):3751-4. 2. Rife JP, et al. N 2-methylguanosine is iso-energetic with guanosine in RNA duplexes and GNRA tetraloops. Nucleic Acids Res. 1998 Aug 15;26(16):3640-4. |