| Description | L-Canavanine sulfate is a selective inducible NO synthase inhibitor. |
| In vitro | L-Canavanine sulfate本身几乎无毒，但能增强VIN和PTX对HeLa细胞和肝细胞癌细胞的细胞毒性[1]。 |
| In vivo | Intravenous infusion of L-canavanine (100 mg/kg), a selective inhibitor of inducible NO synthase in vitro, 60 min and 180 min after lipopolysaccharide challenge, produced an increase in mean arterial pressure and reversed the lipopolysaccharide induced hypotension. However, in lipopolysaccharide challenged animals protected from hypotension by administration of L-canavanine (60 min post challenge), intravenous infusion of NG-nitro-L-arginine methyl ester at 180 min post challenge caused an immediate rise in mean arterial pressure, followed by a rapid fall in blood pressure and heart rate, and sudden death. In contrast, a second dose of L-canavanine at 180 min post challenge maintained blood pressure for the duration of the experiment. Inhibition of both constitutive and inducible NO synthase during endotoxaemia is lethal[2]. |
| Cell experiments | The following cancer cells grown in arginine-rich and arginine-free media were employed: HeLa, Hep G2 and SK-HEP-1. Drug combination experiment used a method based on the median-effect principle and mass-action law[1]. |
| molecular weight | 274.25 |
| Molecular formula | C5H14N4O7S |
| CAS | 2219-31-0 |
| Storage | Powder: -20°C for 3 years | In solvent: -80°C for 1 year |
| Solubility | H2O: 100 mg/mL (364.63 mM), Sonication and heating are recommended. |
| References | 1. Nurcahyanti A D R , Wink M . Cytotoxic potentiation of vinblastine and paclitaxel by L-canavanine in human cervical cancer and hepatocellular carcinoma cells[J]. Phytomedicine, 2015, 22(14):S0944711315003190. 2. Teale D M , Atkinson A M . L-Canavanine restores blood pressure in a rat model of endotoxic shock[J]. European Journal of Pharmacology, 1994, 271(1):0-92. |