| Description | Ganoderol A has significant anti-inflammatory activity and protection against UVA damage. it has an inhibitory effect on angiotensin converting enzyme activity and cholesterol biosynthesis. |
| In vitro | The cytotoxicity and in vitro protective effect of Ganoderol A against UVA damage were evaluated by MTT assay. Apoptosis and cell-cycle arrest of NIH/3T3 fibroblast cells were assayed by fluorescence-activated cell sorting (FCS). Expression of monocyte chemotactic protein-1 (MCP-1) and inducible nitric oxide synthase (iNOS) were determined using quantitative real-time polymerase chain reaction (qPCR). Results: The results indicate that the maximal non-toxic concentration of Ganoderol A in NIH/3T3 cells and RAW 264.7 macrophages was 50 and 25 μg/mL respectively. DNA in the tails and tail length decreased by 55 and 70%, respectively, in the group pretreated with Ganoderol A compared with the UVA-treated group. G1 phase cells decreased by 23%, whereas the number of apoptotic cells returned to normal. The expression of MCP-1 and iNOS declined to 60 and 15%, respectively, compared with LPS-stimulated group. |
| Synonyms | 灵芝醇 A, 灵芝醇A |
| molecular weight | 438.69 |
| Molecular formula | C30H46O2 |
| CAS | 104700-97-2 |
| Storage | Powder: -20°C for 3 years | In solvent: -80°C for 1 year |
| References | 1. In vitro Protective Effect of Ganoderol A Isolated from Ganadermalucidum Against Ultraviolet A Radiation and its Anti-inflammatory PropertiesTrop. J. Pharm. Res., 2015, 14(3):412-21. |