| Description | Fuziline is a diterpene alkaloid found in Aconitum kusnezoffii and Aconitum carmichaelii.It is functionally related to an aconitane. |
| In vitro | In the in vitro assays,fuziline increases cell viability in the model of ISO‐induced injury. |
| Cell experiments | Rat cardiomyocytes(H9c2）were cultured in 96-well plates with High‐glucose DMEM added with 10% FBS,100 μg/mL streptomycin and 100 U/mL penicillin,and H9c2 cells were placed in an incubator with 37°C and 5% CO2 for 24 hours. then addressed with isoproterenol(ISO) and different concentrations of fuziline for the indicated time. Then, the DMEM was removed and 100 μL MTT solution (0.1 mg/well) was added, and H9c2 cells were incubated for 3 hours. After that, the supernatant was removed and the formazan crystals were dissolved in 150 μL DMSO. Then, the absorbance of each sample was detected at 490 nm by a microplate reader.[2] |
| molecular weight | 453.57 |
| Molecular formula | C24H39NO7 |
| CAS | 80665-72-1 |
| Storage | Powder: -20°C for 3 years | In solvent: -80°C for 1 year |
| Solubility | DMSO: 50 mg/mL (110.24 mM) |
| References | 1. Liang X , Cheng P , Xiao-Fang X , et al. Alkaloids Isolated from the Lateral Root of Aconitum carmichaelii[J]. Molecules, 2012, 17(8):9939-9946. 2. Fan CL, et al. Fuziline alleviates isoproterenol-induced myocardial injury by inhibiting ROS-triggered endoplasmic reticulum stress via PERK/eIF2α/ATF4/Chop pathway. J Cell Mol Med. 2020 Jan;24(2):1332-1344. |