| Description | Eugenol (Allylguaiacol) is a Standardized Chemical Allergen. The physiologic effect of eugenol is by means of Increased Histamine Release and Cell-mediated Immunity. |
| In vitro | Eugenol 在多种癌细胞株及B16黑色素瘤异种移植模型中表现出抗增殖效果。对包括肥大细胞、黑色素瘤细胞和HL-60白血病细胞在内的各种癌细胞，Eugenol 能诱导凋亡。在低剂量（2 μM）下，Eugenol 对不同乳腺癌细胞显示出特异性毒性。这种杀伤效应主要通过诱导内源性凋亡途径以及强烈下调E2F1及其下游抗凋亡靶蛋白survivin实现，这一过程不受p53和ERα状态的影响。Eugenol 还能抑制多个与乳腺癌相关的癌基因，如NF-κB和cyclin D1。此外，Eugenol 能上调多功能的细胞周期依赖性激酶抑制蛋白p21WAF1，并以p53独立方式抑制乳腺癌细胞的增殖[2]。 |
| In vivo | Eugenol 不仅在体内抑制乳腺癌细胞的增殖，其对癌蛋白的抑制效果也在肿瘤异种移植物中得到了观察[2]。口服Eugenol （0.2、1.0、5.0或25 mg/kg）分别在四氯化碳（CCl4）剂量（腹腔注射0.4 mg/kg）前（-1小时）、同时（0小时）或后（+3小时）三种不同时间给药时，能显著阻止血清谷丙转氨酶（SGOT）活性升高、脂质过氧化以及肝坏死。在1 mg和5 mg的Eugenol 剂量下，保护效果更为显著，而0.2 mg和25 mg剂量的效果则相对减弱。然而，Eugenol 并不能阻止CCl4处理导致的微粒体葡萄糖-6-磷酸酶（G-6-pase）活性下降，表明Eugenol 无法保护内质网的损伤[3]。 |
| Cell experiments | Cells are seeded into 96-well plates and incubated overnight. The medium is replaced with fresh one containing the desired concentrations of eugenol. After 20 hrs, 10 μl of the WST-1 reagent is added to each well and the plates are incubated for 4 hrs at 37°C. The amount of formazan is quantified using ELISA reader at 450 nm of absorbance.(Only for Reference) |
| Synonyms | 丁香酚, 4-Allylguaiacol, Allylguaiacol, 4-Allyl-2-methoxyphenol, Eugenic acid |
| molecular weight | 164.2 |
| Molecular formula | C10H12O2 |
| CAS | 97-53-0 |
| Storage | Powder: -20°C for 3 years | In solvent: -80°C for 1 year |
| Solubility | DMSO: 65 mg/mL (395.85 mM) |
| References | 1. Nagababu E, et al. Free Radic Res. 1995, 23(6):617-27. 2. Al-Sharif I, et al. BMC Cancer. 2013, 13:600. 3. Nagababu E, et al. Methods Mol Biol. 2010, 610:165-80. |