| Description | (-)-Epigallocatechin Gallate (EGCG) is a natural product of tea flavonoids, which can inhibit EGFR signaling and GLUD 1/2. (-)-Epigallocatechin Gallate has antioxidant, anti-inflammatory, and antitumor activities. (-)-Epigallocatechin Gallate can induce oxidative phosphorylation by activating cytochrome c oxidase. |
| In vitro | 方法：人甲状腺癌细胞 TT、TPC-1 和 ARO 用 (-)-Epigallocatechin Gallate (10-200 µM) 处理 24 h，使用 MTS assay 检测细胞活力。结果：(-)-Epigallocatechin Gallate 以剂量依赖的方式抑制 TT、TPC-1 和 ARO 细胞的活力。[1]方法：新生儿人类真皮成纤维细胞 nHDFs 用 (-)-Epigallocatechin Gallate (100-800 µmol/L) 处理 24 h，使用 Annexin V-FITC assay 检测细胞凋亡。结果：在低于 200 µmol/L (-)-Epigallocatechin Gallate 的剂量下，既没有观察到细胞凋亡，也没有观察到坏死。然而，用更高浓度的 (-)-Epigallocatechin Gallate 处理 nHDFs 导致晚期细胞凋亡和坏死。[2] |
| In vivo | 方法：为研究对急性损伤的保护作用，将 (-)-Epigallocatechin Gallate (200 mg/kg) 灌胃给药给 ICR 小鼠，每天一次，持续一周，之后腹腔注射 LPS (15 mg/kg) 诱导急性损伤。结果：(-)-Epigallocatechin Gallate 可以保护小鼠免受 LPS 诱导的急性应激，同时稳定肠道微生物，改善鞘脂代谢，抑制有害代谢产物的含量。[3] |
| Cell experiments | LoVo, SW480, HCT-8, and HT-29 cells are seeded in 96-well plates at a concentration of 5×103?cells; each cell line is totally seeded in the 12 wells. Complete medium is added to the wells, up to 200 μL; the medium contains 0 μg/mL, 10 μg/mL, 20 μg/mL, and 35 μg/mL of epigallocatechol gallate. The inhibition rate=[1 - (absorbance of Epigallocatechol Gallate group - absorbance of control group)/(absorbance of control group - absorbance of blank control group)] × 100. |
| Synonyms | Epigallocatechol Gallate, (-)-表没食子儿茶素没食子酸酯, EGCG |
| molecular weight | 458.37 |
| Molecular formula | C22H18O11 |
| CAS | 989-51-5 |
| Storage | Powder: -20°C for 3 years | In solvent: -80°C for 1 year |
| Solubility | DMSO: 100 mg/mL (218.16 mM) H2O: 50 mg/mL (109.08 mM), Sonication is recommended. Ethanol: 45.8 mg/mL (100 mM) |
| References | 1. Wu D, et al. Epigallocatechin-3-gallate inhibits the growth and increases the apoptosis of human thyroid carcinoma cells through suppression of EGFR/RAS/RAF/MEK/ERK signaling pathway. Cancer Cell Int. 2019 Feb 28;19:43. 2. Han DW, et al. Epigallocatechin-3-gallate regulates cell growth, cell cycle and phosphorylated nuclear factor-κB in human dermal fibroblasts. Acta Pharmacol Sin. 2011 May;32(5):637-46. 3. Ma Y, et al. Epigallocatechin Gallate Can Protect Mice From Acute Stress Induced by LPS While Stabilizing Gut Microbes and Serum Metabolites Levels. Front Immunol. 2021 Apr 1;12:640305. 4. Jin H, et al. Epigallocatechin gallate inhibits the proliferation of colorectal cancer cells by regulating Notch signaling. Onco Targets Ther. 2013;6:145-53. 5. Lu J, Lu Z, Liu L, et al. Identification of crocin as a new hIAPP amyloid inhibitor via a simple yet highly biospecific screening system[J]. Chemistry & Biodiversity. 2021 6. Zhou H, Ning Y, Zeng G, et al. Curcumin promotes cell cycle arrest and apoptosis of acute myeloid leukemia cells by inactivating AKT[J]. Oncology Reports. 2021, 45(4): 1-1. |