| Description | Endomorphin 1 is a high affinity and selective agonist of the μ-opioid receptor and displays reasonable affinities for kappa3 binding sites (Ki: 20~30 nM). |
| In vitro | Endomorphin是一种内源性阿片肽，也是两种Endomorphins之一。它是μ-阿片受体的高亲和力、高选择性激动剂，并且与Endomorphin 2一同作用。Endomorphin 1和Endomorphin 2都能强效地竞争μ1与μ2受体位点。Endomorphins对delta或kappa1结合位点的亲和力很低，其Ki值均大于500 nM。 |
| In vivo | Endomorphin 1和Endomorphin 2均为有效的镇痛剂，其效果分别在10分钟和15分钟达到顶峰。所有后续研究均在达到最大效应时进行。这两种化合物在脊髓上方和脊髓内均完全活跃，且没有出现效应上限。Endomorphin 1和Endomorphin 2的作用特征与吗啡相似。在对CD-1小鼠产生超过70%镇痛效果的剂量下，这两种化合物在CXBK小鼠中均无镇痛活性。 |
| Animal experiments | Groups of mice are treated i.c.v. with Endomorphin 1 (12 μg) or Endomorphin 2 (3 μg) 15 min before a 0.5-cc charcoal meal (2.5% gum tragacanth,10% activated charcoal in water). The mice are killed 30 min later and the distance the charcoal traveled is measured. |
| Target activity | κ3 opioid receptor:20-30 nM (Ki) |
| Synonyms | 内吗啡肽 1, Endomorphin-1 |
| molecular weight | 610.7 |
| Molecular formula | C34H38N6O5 |
| CAS | 189388-22-5 |
| Storage | keep away from moisture | Powder: -20°C for 3 years | In solvent: -80°C for 1 year |
| Solubility | H2O: 24 mg/mL (39.3 mM), Sonication is recommended. |
| References | 1. Goldberg IE, et al. Pharmacological characterization of endomorphin-1 and endomorphin-2 in mouse brain. J Pharmacol Exp Ther. 1998 Aug;286(2):1007-13. |
| Kinase | 125I-Endomorphin 1 or 125I-Endomorphin 2 binding (0.2 nM) is performed in potassium phosphate buffer (50 mM, pH 7.4; 0.5 mL) with MgCl2 (5 mM) at a tissue concentration of 10 mg wet weight/mL for brains or 0.06 mg protein/mL for MOR-1/CHO cells. Specific binding is determined in the presence and absence of either 1 μM of the corresponding unlabeled peptide. The entire mixture is then incubated at 25°C for 1 hr and filtered over no. 32 glass fiber filters which have been presoaked for 1 hr in 0.5% polyethyleneimine and washed twice with ice-cold Tris buffer using a Brandel cell harvester. The filters are then counted on a Packard Cobra gamma counter. The other opioid receptor binding assays are performed. |