| Description | D-Galactose (Alpha-D-galactose) is a naturally occurring hexose sugar that combines with glucose to form lactose. D-Galactose is often used in animal aging models. |
| In vitro | 方法：星形细胞 CRT 和大鼠原代星形胶质细胞用 D-Galactose (10-100 g/L) 处理 72 h，使用 WST-1 assay 检测细胞活力。结果：40 g/L 以上的 D-Galactose 以剂量依赖性方式显著抑制细胞活力。[1]方法：胶质母细胞瘤细胞系 C6 用 D-Galactose (28-222 mM) 处理 8 天，使用 Western Blot 检测靶点蛋白表达水平。结果：与对照组相比，D-Galactose 处理组中裂解的 cleaved-caspase-3/caspase-3 水平显著降低。[2] |
| In vivo | 方法：为研究对动物行为的影响，将 D-Galactose (50-200 mg/kg) 皮下注射给 C57 小鼠构建衰老模型，每天一次，持续八周。结果：D-Galactose 可在 50-100 mg/kg 范围内以剂量依赖性方式诱导 C57 小鼠的行为损伤，高于 100 mg/kg 的剂量不会进一步恶化其行为。[3] |
| Synonyms | D-(+)-Galactose, alpha-D-半乳糖, D-半乳糖, D-Galactopyranose, Alpha-D-galactose |
| molecular weight | 180.16 |
| Molecular formula | C6H12O6 |
| CAS | 59-23-4 |
| Storage | Powder: -20°C for 3 years | In solvent: -80°C for 1 year |
| Solubility | H2O: 66mg/mL（366.34mM） DMSO: 55 mg/mL (305.28 mM) |
| References | 1. Hou J, et al. D‑galactose induces astrocytic aging and contributes to astrocytoma progression and chemoresistance via cellular senescence. Mol Med Rep. 2019 Nov;20(5):4111-4118. 2. Xu X, et al. D-galactose induces senescence of glioblastoma cells through YAP-CDK6 pathway. Aging (Albany NY). 2020 Sep 29;12(18):18501-18521. 3. Wei H, et al. Behavioural study of the D-galactose induced aging model in C57BL/6J mice. Behav Brain Res. 2005 Feb 28;157(2):245-51. |