| Description | Cerebroside B1b has antiulcerogenic activity. |
| In vitro | Here, fusaruside is clarified biosynthetically, and its efficacy-related 10,11-double bond can be generated under the regioselective catalysis of an unprecedented α?10(E)-sphingolipid desaturase (α?10(E)-SD). α?10(E)-SD shares 17.7% amino acid sequence similarity with a C9-unmethylated α?10-sphingolipid desaturase derived from a marine diatom, and 55.7% with α?8(E)-SD from Fusarium graminearum. Heterologous expression of α?10(E)-SD in Pichia pastoris has been established to facilitate a reliable generation of 1 through the α?10(E)-SD catalyzed desaturation of Cerebroside B (2), an abundant fungal sphingolipid. Site directed mutageneses show that the conserved histidines of α?10(E)-SD are essential for the 10,11-desaturation catalysis, which is also preconditioned by the C9-methylation of the substrate. Moreover, α?10(E)-SD confers improved survival and faster growth to fungal strains at low temperature and high salinity, in parallel with to higher contents of 1 in the mycelia. |
| molecular weight | 728.065 |
| Molecular formula | C41H77NO9 |
| CAS | 88642-46-0 |
| Storage | Powder: -20°C for 3 years | In solvent: -80°C for 1 year |
| References | 1. α10(E)-Sphingolipid Desaturase Involved in Fusaruside Mycosynthesis and Stress Adaptation in Fusarium graminearum.Sci Rep. 2015 May 21;5:10486. |