| Description | Berberine (Umbellatine) is a natural product of alkaloids, which can activate AMPK, inhibit DNA topoisomerase, and induce ROS generation. Berberine has anti-tumor, anti-bacterial, hypoglycemic, hypolipidemic and other biological activities. |
| In vitro | 方法：人骨肉瘤细胞 MG-63 用 Berberine (20-80 µM) 处理 12-24 h，使用 MTT assay 检测细胞活力。结果：Berberine 诱导 MG-63 细胞活力的浓度和时间依赖性降低。[1]方法：NPC 细胞系 HONE1 用 Berberine (25-50 µM) 处理 24 h，使用 Western Blot 检测靶点蛋白表达水平。结果：Berberine 可以有效抑制 HONE1 细胞中 p-STAT3 的水平。STAT3 激活的下调与 Mcl-1 的表达受到抑制和 cleaved PARP-1 水平增加有关。[2] |
| In vivo | 方法：为测试体内抗肿瘤活性，将 Berberine (5-10 mg/kg) 腹腔注射给携带 NPC 肿瘤 C666-1 的 nude 小鼠，每两天一次，持续三十天。结果：Berberine 能有效抑制 EBV 阳性 NPC 细胞系 (C666-1) 在裸鼠体内的致瘤性和生长。抑制体内 NPC 细胞的致瘤性生长与有效抑制裸鼠肿瘤异种移植物内 NPC 细胞中 STAT3 的激活相关。[2] |
| Synonyms | 小檗碱, Berberin, Umbellatine, 黄连素 |
| molecular weight | 336.36 |
| Molecular formula | C20H18NO4 |
| CAS | 2086-83-1 |
| Storage | Powder: -20°C for 3 years | In solvent: -80°C for 1 year |
| Solubility | Chloroform, Dichloromethane, Ethyl Acetate, Acetone, etc.: Soluble DMSO: 5.63 mg/mL (16.72 mM), Sonication is recommended. |
| References | 1. Zhu Y, et al. Berberine induces apoptosis and DNA damage in MG‑63 human osteosarcoma cells. Mol Med Rep. 2014 Oct;10(4):1734-8. 2. Tsang CM, et al. Berberine suppresses tumorigenicity and growth of nasopharyngeal carcinoma cells by inhibiting STAT3 activation induced by tumor associated fibroblasts. BMC Cancer. 2013 Dec 31;13:619. 3. Zhou Z, Lin Y, Gao L, et al. Circadian Pharmacological Effects of Berberine on Chronic Colitis in Mice: Role of the Clock Component Rev-erbα[J]. Biochemical Pharmacology. 2020, 172: 113773. |