| Description | Baicalein (5,6,7-Trihydroxyflavone) belongs to the flavonoid group of natural products and is a xanthine oxidase inhibitor, a CYP2C9 inhibitor, and a prolyl endopeptidase inhibitor. Baicalein has antitumor, anti-inflammatory, and antibacterial activities. |
| In vitro | 方法：人乳腺癌细胞 MDA-MB-231 用 Baicalein (0-120 µmol/L) 处理 24-72 h，使用 MTT assay 检测细胞活力。结果：Baicalein 对 MDA-MB-231 细胞的增殖潜力具有剂量依赖性抑制作用。Baicalein 在 24、48 和 72 h 的 IC50 分别为 89.71、59.50 和 33.61 µmol/L。[1]方法：滤泡型未分化甲状腺癌细胞 FRO 用 Baicalein (10-80 µM) 处理 36-48 h，使用 Flow cytometry 检测细胞凋亡。结果：各剂量 Baicalein 均能显著增加 FRO 细胞的凋亡，且呈剂量依赖性。[2] |
| In vivo | 方法：为研究体内对肿瘤转移的影响，将 Baicalein (50-100 mg/kg) 灌胃给药人乳腺癌细胞 MDA-MB-231 的裸鼠移植模型，每天一次，持续十五天。结果：Baicalein 在体内抑制肿瘤转移。Baicalein 在体内下调 SATB1、EMT 和 Wnt/β-catenin 通路的表达。[1] |
| Cell experiments | MTT assay is conducted to evaluate the effect of baicalein on proliferation of breast cancer cells. MDA-MB-231 cells are routinely digested, collected, and then seeded in 96-well plates at a density of 8×103 cells/well. After incubation for 12-24 hours, cells are treated with 0, 20, 40, 60, 80, 100, and 120 μM baicalein according to their experimental grouping and then incubated at 37°C for 24, 48, and 72 hours[3]. |
| Target activity | XO:3.12 mM. |
| Synonyms | 5,6,7-Trihydroxyflavone, 黄芩素, 黄芩黄素 |
| molecular weight | 270.24 |
| Molecular formula | C15H10O5 |
| CAS | 491-67-8 |
| Storage | Powder: -20°C for 3 years | In solvent: -80°C for 1 year |
| Solubility | DMSO: 50 mg/mL (185.02 mM) |
| References | 1. Ma X, et al. Baicalein suppresses metastasis of breast cancer cells by inhibiting EMT via downregulation of SATB1 and Wnt/β-catenin pathway. Drug Des Devel Ther. 2016 Apr 18;10:1419-41. 2. Wang M, et al. Baicalein induced apoptosis and autophagy of undifferentiated thyroid cancer cells by the ERK/PI3K/Akt pathway. Am J Transl Res. 2019 Jun 15;11(6):3341-3352. 3. Kong EK, et al. A novel anti-fibrotic agent, baicalein, for the treatment of myocardial fibrosis in spontaneously hypertensiverats. Eur J Pharmacol. 2011 May 11;658(2-3):175-81. 4. Meng X, Mao Z, Li X, et al. Baicalein decreases uric acid and prevents hyperuricemic nephropathy in mice[J]. Oncotarget. 2017, 8(25): 40305. |