| Description | Ascamycin is a 5'-O-sulfonamide ribonucleoside antibiotic produced by Streptomyces spp. It shows selective antibacterial activity against Xanthomonas species and inhibits Xanthomonas citri, Xanthomonas oryzae and phage with MIC values of 0.4 μg/mL, 12.5 μg/mL and 12.5 μg/mL respectively. |
| In vitro | Ascamycin has a selective antibacterial activity against Xanthomonas species and it has C2-chloroadenine as the base on C-1' which lacks the chlorine.[1] Xanthomonas citri is susceptible to Ascamycin by virtue of the Ascamycin-dealanylating enzyme on the cell surface, When Ascamycin is dealanylated, Dealanylascamycin shows a broad antibacterial activity against various Gram-negative and Gram-positive bacteria.[2] |
| Target activity | Xanthomonas citri:（MIC）0.4 μg/ml, Xanthomonas oryzae:12.5 μg/ml, Mycobacterium phlei:12.5 μg/ml, Xanthomonas citri:0.4 μg/ml(MIC), Xanthomonas oryzae:12.5 μg/ml(MIC), Mycobacterium phlei:12.5 μg/ml(MIC) |
| molecular weight | 451.84 |
| Molecular formula | C13H18ClN7O7S |
| CAS | 91432-48-3 |
| Storage | Powder: -20°C for 3 years | In solvent: -80°C for 1 year |
| Solubility | DMSO: 10 mg/mL (22.13 mM) |
| References | 1. Isono K, et al. Ascamycin and dealanylascamycin, nucleoside antibiotics from Streptomyces sp. J Antibiot (Tokyo). 1984 ; 37(6):670-672. 2. Osada H, rt al. Purification and characterization of ascamycin-hydrolysing aminopeptidase from Xanthomonas citri. Biochem J. 1986 ; 233(2):459-463. 3. Zhao C, et al. Characterization of biosynthetic genes of ascamycin/dealanylascamycin featuring a 5'-O-sulfonamide moiety in Streptomyces sp. JCM9888. PLoS One. 2014 ; 9(12):e114722. |