| Description | 7-Epi 10-desacetyl paclitaxel (10-Deacetyl-7-epipaclitaxel) shows IC50 values of 0.085 nM, against HeLa cells. |
| In vitro | 7-Epi-10-deacetyltaxol(EDT) exhibited significant in vitro cytotoxicity in HepG2 cells.Nuclear condensation and DNA fragmentation were observed in cells treated with fungal EDT.?Besides, the fungal EDT arrested HepG2 cells at G2/M phase of cell cycle.?Furthermore, fungal EDT induced apoptosis in HepG2 cells in a dose-dependent manner associated with ROS generation and increased Bax/Bcl-2 ratio, p38 MAPKs and PARP cleavage[1]. |
| Cell experiments | The endophytic fungus was identified by traditional and molecular taxonomical characterization and the fungal 7-Epi-10-deacetyltaxol(EDT) was purified using column chromatography and confirmed by various spectroscopic and chromatographic comparisons with authentic paclitaxel.?Studied the in vitro effects of EDT on HepG2 cells for parameters such as cell cycle distribution, DNA fragmentation, reactive oxygen species (ROS) generation and nuclear morphology.?Further, western blot analysis was used to evaluate Bcl-2-associated X protein (Bax), B-cell lymphoma 2 (Bcl-2), p38-mitogen activated protein kinase (MAPK) and poly [ADP-ribose] polymerase (PARP) expression[1]. |
| Synonyms | 10-Deacetyl-7-epipaclitaxel, 7-Epi-10-deacetyltaxol, 7-表-10-去乙酰基云南紫杉宁A |
| molecular weight | 811.87 |
| Molecular formula | C45H49NO13 |
| CAS | 78454-17-8 |
| Storage | Powder: -20°C for 3 years | In solvent: -80°C for 1 year |
| Solubility | DMSO: 8.11 mg/mL (9.99 mM) |
| References | 1. Subban K , Singh S , Subramani R , et al. Fungal 7-epi-10-deacetyltaxol produced by an endophytic Pestalotiopsis microspora induces apoptosis in human hepatocellular carcinoma cell line (HepG2)[J]. BMC Complementary and Alternative Medicine, 2017, 17(1):504. 2. Dang P H , Nguyen H X , Duong T T T , et al. α-Glucosidase Inhibitory and Cytotoxic Taxane Diterpenoids from the Stem Bark of\r, Taxus wallichiana[J]. Journal of Natural Products, 2017:acs.jnatprod.7b00006. |