| Description | 5,6-Dihydrouridine is a modified base found in conserved positions in the D-loop of tRNA in Eukaryota, Bacteria, and some Archaea. |
| In vitro | 5,6-Dihydrouridine (Dihydrouridine) is one of the posttranscriptionally modified nucleosides. It is a product of the reduction of uridine (U) and can be further modified to 5-methyldihydrouridine (m5D). It is identified in six positions in the 'D-loop” of the tRNA (16, 17, 20a, 20b) and in position 47 in the variable loop. |
| Target activity | Cytidine deaminase (E. coli):3.4 μM(Ki) |
| molecular weight | 246.22 |
| Molecular formula | C9H14N2O6 |
| CAS | 5627-05-4 |
| Storage | Powder: -20°C for 3 years | In solvent: -80°C for 1 year |
| Solubility | H2O: 90.0 mg/mL (365.5 mM), Sonication is recommended. DMSO: 32.1 mg/mL (130.5 mM), Sonication is recommended. |
| References | 1. Kasprzak JM, et al. Molecular evolution of dihydrouridine synthases. BMC Bioinformatics. 2012 Jun 28;13:153. 2. Cohen RM, et al Cytidine deaminase from Escherichia coli. Purification, properties and inhibition by the potential transition state analog 3,4,5,6-tetrahydrouridine. J Biol Chem. 1971;246(24):7561-7565. |