| Description | 4-Methoxycinnamyl alcohol showed toxicity to MCF-7, HeLa and DU145 cancer cell lines with IC50 values of 14.24, 7.82 and 22.10 μg/mL, respectively. 4-Methoxycinnamyl alcohol was isolated from Foeniculum vulgare. 4-Methoxycinnamyl alcohol did not show apoptotic effects but showed necrosis after 48 h in a 10 μg/mL DNA fragmentation study. |
| In vitro | 在本研究中，通过评估己烷、乙酸乙酯和水相分的E. pavieana根茎乙醇提取物对LPS刺激的巨噬细胞产生NO的抑制作用及其机制，探讨了其抗炎效果。在LPS刺激的RAW264.7巨噬细胞中进行了NO产生的抑制活动测试。植物提取物的细胞毒性通过3-(4,5-二甲基噻唑-2-基)-2,5-二苯基四唑溴化物(MTT)测定，mRNA和蛋白表达量则分别通过逆转录聚合酶链反应(RT-PCR)和西方印迹分析进行测定。乙酸乙酯分部（EPE）显示出对巨噬细胞NO产生最强的抑制效果。EPE显著降低NO产生，并以剂量依赖性方式抑制诱导型一氧化氮合酶(iNOS)蛋白和mRNA的表达。此外，处理EPE的活化细胞中核因子-κB p65亚单位水平显著降低。通过活性导向分离技术获得了四种酚类化合物，分别是4-Methoxycinnamyl alcohol（1）、反式4-甲氧基肉桂醛（2）、4-甲氧基肉桂基对香豆酸酯（3）和对香豆酸（4）。结论：E. pavieana根茎提取物中所含的抗炎性质通过抑制iNOS表达及NO生成，为基于该植物提取物开发新的抗炎剂提供了科学依据和支持。[1] |
| Target activity | MCF-7 cell lines:14.24 μg/mL, HeLa cells:7.82 μg/mL, DU145 cell lines:22.10 μg/mL |
| molecular weight | 164.2 |
| Molecular formula | C10H12O2 |
| CAS | 53484-50-7 |
| Storage | store at low temperature | Powder: -20°C for 3 years | In solvent: -80°C for 1 year |
| References | 1. Srisook E, et al. Anti-inflammatory Effect of Etlingera pavieana (Pierre ex Gagnep.) R.M.Sm. Rhizomal Extract and Its Phenolic Compounds in Lipopolysaccharide-Stimulated Macrophages. Pharmacogn Mag. 2017;13(2):230-235. 2. Lall N, et al. Cytotoxicity of syringin and 4-methoxycinnamyl alcohol isolated from Foeniculum vulgare on selected human cell lines. Nat Prod Res. 2015;29(18):1752-1756. |