| Description | 25R-Inokosterone exhibits potent inhibition against TNF-expression levels in A23187 plus phorbol-myrisrate acetate-induced RBL-2H3 cells. 25R-Inokosterone has excellent anti-atopy activity, thus 25R-Inokosterone could be used to a large range of functional anti-atopy cosmetics. |
| In vitro | Three compounds were isolated from the subfraction 5 through the repeated prep- high performance liquid chromatography column chromatography. According to the results of physico-chemical and spectroscopic data including NMR and MS, the chemical structures of the compounds were determined as ecdysterone (1), 25S-inokosterone (2), and 25R-Inokosterone (3). Three phytoecdysones were showed weak inhibitory activity for thymus and activation-regulated chemokine expression levels in tumor necrosis factor (TNF)-α± plus IFN-γ induced HaCaT cells, respectively. However, those compounds 1-3 were exhibited the most potent inhibition (80??95% at 200 μg/mL) against TNF-α± expression levels in A23187 plus phorbol-myrisrate acetate-induced RBL-2H3 cells. |
| molecular weight | 480.642 |
| Molecular formula | C27H44O7 |
| CAS | 19682-38-3 |
| Storage | Powder: -20°C for 3 years | In solvent: -80°C for 1 year |
| References | 1. Phytoecdysones from the Roots of Achyranthes japonica Nakai and their Anti-atopy ActivityJournal of Applied Biological Chemistry, 2015 , 58 (1) :13-19. |