| Bioactivity | sBADA TFA is a potent green fluorescent dye. sBADA TFA is a sulfonated BODIPY-FL 3-amino-D-alanine. sBADA TFA is used to label peptidoglycans in bacterial cell walls in situ. (λex=490 nm, λem=510 nm)[1]. |
| Invitro | Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs).sBADA TFA assay[1] (to quantify GAPDH):1. Wash S. venezuelae cells twice with pre‐warms fresh LB broth (30°C) and collects via centrifuge (7000 g, 2 minutes).2. S. venezuelae cells are re-suspended in fresh LB broth (30°C) containing 1mM sBADA TFA and cultured for 5 minutes.3. S. venezuelae cells are treated with 70% ice-cold ethanoland incubates on ice-bath for1 hour.4. The fixed cells are then washed twice with 4°C 1x PBS via centrifuge and re‐suspends in 4°C 1x PBS before imaging. |
| Name | sBADA TFA |
| Formula | C19H21BF5N4NaO8S |
| Molar Mass | 594.25 |
| Transport | Room temperature in continental US; may vary elsewhere. |
| Storage | Please store the product under the recommended conditions in the Certificate of Analysis. |
| Reference | [1]. Hsu YP, et, al. Full color palette of fluorescent d-amino acids for in situ labeling of bacterial cell walls. Chem Sci. 2017 Sep 1;8(9):6313-6321. |