| Bioactivity | o-Pah is a BODIPY derivative with an -NH2 and -OH substituted meso-Ph group. o-Pah exhibits metal-induced J-aggregation in the presence of Cu2+ and a specific fluorescence enhancement for Hg2+ (Ex/Em=483/(495-600) nM)[1]. |
| Invitro | o-Pah (20 μM) shows changed colour upon addition of 100 μM different metal ions and the further addition of 20 μM of Cu2+ in DMSO/HEPES buffer solution[1].Procedures of metal ion sensing Stock solutions of the metal ions (100 mM and 10 mM) were prepared in deionised water[1].1. A stock solution of o-Pah (0.5 mM) is prepared in DMSO.2. The solution of o-Pah is then diluted to 5 μM with HEPES buffer solution (50 mM KNO3, 50 mM HEPRS, pH 7.2).3. During titration experiments, 2 mL solutions of o-Pah (5 μM, 0.5 μM) are placed in a 1 cm quartz optical cell and Cu2+, Hg2+ stock solutions are added gradually with a micropipette.4. In selectivity experiments, the test samples are prepared by adding appropriate amounts of the metal ion stock solution into the 2 mL solution of o-Pah (5 μM).6. During fluorescence measurements, the excitation wavelength is 483 nm and emission spectra are collected between 495-600 nm. |
| Name | o-Pah |
| CAS | 1181844-41-6 |
| Formula | C19H20BF2N3O |
| Molar Mass | 355.19 |
| Transport | Room temperature in continental US; may vary elsewhere. |
| Storage | Please store the product under the recommended conditions in the Certificate of Analysis. |
| Reference | [1]. Lu H, et al. Specific Cu(2+)-induced J-aggregation and Hg(2+)-induced fluorescence enhancement based on BODIPY. Chem Commun (Camb). 2010 May 28;46(20):3565-7. |