Z-Gly-Pro-AMC_product_DataBase

Bioactivity	Z-Gly-Pro-AMC is a fluorogenic substrate. Z-Gly-Pro-AMC is hydrolyzed by prolyl endopeptidase to generate highly fluorescent 7-amido-4-methylcoumarin (HY-D0027). (λex=380 nm, λem=465 nm)[1].
Invitro	Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs).Z-Gly-Pro-AMC assay[1]:1. Make a 5 μL of plasma sample is pre-incubated with 10 μL of 250 nM FAP inhibitor, 10 μL of 250 nM PREP inhibitor or 10 μL of 0.0025% (v/v) DMSO for 15 min at 37 ℃.2. Add 35 μL pre-heated Z-Gly-Pro-AMC (380 μM diluted in buffer) to obtain a final concentration of 266 μM.3. Fluorescence is measured kinetically for 30 min at 37 ℃.
Name	Z-Gly-Pro-AMC
CAS	68542-93-8
Formula	C25H25N3O6
Molar Mass	463.48
Appearance	Solid
Transport	Room temperature in continental US; may vary elsewhere.
Storage	-20°C, protect from light, stored under nitrogen *In solvent : -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen)
Reference	[1]. Bracke A, et, al. The development and validation of a combined kinetic fluorometric activity assay for fibroblast activation protein alpha and prolyl oligopeptidase in plasma. Clin Chim Acta. 2019 Aug;495:154-160.

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