| Bioactivity | Steppogenin is a potent inhibitor of HIF-1α and DLL4, with IC50 values of 0.56 and 8.46 μM, respectively. Steppogenin can be sued for the research of angiogenic diseases, such as those involving solid tumors[1]. |
| Target | IC50: 0.56 ± 0.043 μM (HIF-1α), 8.46 ± 1.08 μM (DLL4) |
| Invitro | Steppogenin (0-10 μM, 24 h) 以剂量依赖性方式抑制 HEK293T 细胞缺氧条件下 HIF-1α 的转录活性和血管内皮细胞 (ECs) 中 VEGF 诱导的 DLL4 表达[1]。Steppogenin (0-3 μM，6 h) 在缺氧条件下抑制 HIF-1α 靶基因（VEGF、GLUT1、CXCR4 和 CA9）的 mRNA 表达[1]。Steppogenin (0-3 μM，16 h) 抑制 HIF-1α 蛋白水平，并抑制 VEGF、CXCR4 和 CA9 的蛋白水平[1]。Steppogenin (0-3 μM，24 h) 抑制缺氧诱导的血管 EC 增殖和迁移以及 VEGF 诱导的 EC 球体发芽[1]。 RT-PCR[1] Cell Line: |
| In Vivo | Steppogenin (2 mg/kg，腹腔注射，一次) 抑制肿瘤生长和血管生成[1]。Steppogenin (2 mg/kg，腹腔注射，一次) 在肝脏和脾脏中的分布最高 (分别为 25.5 倍和 9.74 倍 AUC 比率)，T1/2 显著更高[1]。Pharmacokinetic Parameters of Steppogenin in male C57BL/6 J mice[1]. |
| Name | Steppogenin |
| CAS | 56486-94-3 |
| Formula | C15H12O6 |
| Molar Mass | 288.25 |
| Transport | Room temperature in continental US; may vary elsewhere. |
| Storage | Please store the product under the recommended conditions in the Certificate of Analysis. |
| Reference | [1]. Cha S, et al. Steppogenin suppresses tumor growth and sprouting angiogenesis through inhibition of HIF-1α in tumors and DLL4 activity in the endothelium. Phytomedicine. 2023 Jan;108:154513. |