| Bioactivity | Sortin1 is a chemical genetic-hit molecule that causes specific mislocalization of plant and yeast-soluble and membrane vacuolar markers.IC50 value:Target: Vacuolar markersin vitro: In Arabidopsis seedlings, application of Sortin1 and -2 led to reversible defects in vacuole biogenesis and root development. Sortin1 was found to redirect the vacuolar destination of plant carboxypeptidase Y and other proteins in Arabidopsis suspension cells and cause these proteins to be secreted. Sortin1 treatment of whole Arabidopsis seedlings also resulted in carboxypeptidase Y secretion, indicating that the drug has a similar mode of action in cells and intact plants [1]. Structure-activity relationship studies conducted in Arabidopsis revealed the structural requirements for Sortin1 bioactivity and demonstrated that overlapping Sortin1 substructures can be used to discriminate between vacuolar-flavonoid accumulations and vacuolar-biogenesis defects [2]. | ||||||||||||
| Name | Sortin1 | ||||||||||||
| CAS | 503837-98-7 | ||||||||||||
| Formula | C26H19NO6 | ||||||||||||
| Molar Mass | 441.43 | ||||||||||||
| Appearance | Solid | ||||||||||||
| Transport | Room temperature in continental US; may vary elsewhere. | ||||||||||||
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| Reference | [1]. Zouhar J, et al. Sorting inhibitors (Sortins): Chemical compounds to study vacuolar sorting in Arabidopsis. Proc Natl Acad Sci U S A. 2004 Jun 22;101(25):9497-501. [2]. Rosado A, et al. Sortin1-hypersensitive mutants link vacuolar-trafficking defects and flavonoid metabolism in Arabidopsis vegetative tissues. Chem Biol. 2011 Feb 25;18(2):187-97. [3]. Orr DJ, et al. 1H NMR-based metabolomics methods for chemical genomics experiments. Methods Mol Biol. 2014;1056:225-39. |