| Bioactivity | SS-RJW100 is a enantiomer of RJW100, which is a racemic agonist of nuclear receptor liver receptor homolog 1 (LRH-1) and steroidogenic factor 1 (SF-1). SS-RJW100 promotes recruitment of coregulator protein fragments in vitro, recruits the transcriptional intermediary factor 2 (Tif2) coactivator to LRH-1. SS-RJW100 diminishes LRH-1 allosteric activation networks, shows poor thermal stability[1][2]. | ||||||||||||
| Target | Ki: 1.2 μM (LRH-1), 30 μM (SF-1) | ||||||||||||
| Invitro | Liver receptor homolog-1 (LRH-1) and steroidogenic factor-1 (SF-1) are closely related nuclear hormone receptors (NR) that play key roles as regulators of metabolism, inflammation, and proliferation[1]. SS-RJW100 (1 nM-1 mM; ) binds LRH-1 and SF-1, with binding affinity Ki values of 1.2 μM (LRH-1), 30 μM (SF-1), respectively[1].SS-RJW100 (30 μM; 24 h) increases LRH-1 transcriptional activity in both wild-type and mutant LRH-1 overexpressed Hela cells, without being affected by mutations[1]. RT-PCR[1] Cell Line: | ||||||||||||
| Name | SS-RJW100 | ||||||||||||
| Formula | C28H34O | ||||||||||||
| Molar Mass | 386.57 | ||||||||||||
| Appearance | Liquid | ||||||||||||
| Transport | Room temperature in continental US; may vary elsewhere. | ||||||||||||
| Storage |
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| Reference | [1]. Mays SG, et al. Enantiomer-specific activities of an LRH-1 and SF-1 dual agonist. Sci Rep. 2020 Dec 17;10(1):22279. [2]. Stec J. Tandem reaction sequences on a zirconocene template[J]. University of Southampton, 2010. |