Bioactivity | SKF 38393 hydrochloride is a selective agonist of the dopamine D1 receptor (D1DR) with an IC50 of 110 nM[1]. |
Target | IC50: 110 nM (D1DR) |
Invitro | SKF-38393 hydrochloride induces a similar change in cytomorphology and increases the levels of media cAMP[2].SKF-38393 hydrochloride (10 μmol/L; 1 hour) induces increased threonine-phosphorylation of DA- and cAMP-regulated phosphoprotein of Mr 32 kD (DARPP-32) in cultured GC cells[2]. Western Blot Analysis[2]. Cell Line: |
In Vivo | SKF-38393 hydrochloride (10 mg/kg; i.p.) blocks the 1-Methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) -induced depletion of glutathione[3].SKF-38393 hydrochloride attenuates MPTP-induced depletion of dopamine[3].SKF-38393 hydrochloride enhances the activity of superoxide dismutase and hence mimics the action of Selegiline[3].SKF-38393 hydrochloride enhances the frequency but not the amplitude of tetrodotoxin-resistant excitatory postsynaptic currents which argues for a presynaptic locus of D1 action[4]. Animal Model: |
Name | SKF 38393 hydrochloride |
CAS | 62717-42-4 |
Formula | C16H18ClNO2 |
Molar Mass | 291.77 |
Appearance | Solid |
Transport | Room temperature in continental US; may vary elsewhere. |
Storage | 4°C, sealed storage, away from moisture *In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture) |
Reference | [1]. Altar CA, et al. Picomolar affinity of 125I-SCH 23982 for D1 receptors in brain demonstrated with digital subtraction auto radiography. J Neurosci. 1987 Jan;7(1):213-222. [2]. Mayerhofer A, et al. Functional Dopamine-1 Receptors and DARPP-32 Are Expressed in Human Ovary and Granulosa Luteal Cells in Vitro. J Clin Endocrinol Metab. 1999 Jan;84(1):257-64. [3]. Muralikrishnan D, et al. SKF-38393, a dopamine receptor agonist, attenuates 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine-induced neurotoxicity. Brain Res. 2001 Feb 23;892(2):241-7. [4]. Bouron A, et al. The D1 dopamine receptor agonist SKF-38393 stimulates the release of glutamate in the hippocampus. Neuroscience. 1999;94(4):1063-70. |