| Bioactivity | S130 is a high affinity, selective inhibitor of ATG4B (a major cysteine protease) with an IC50 of 3.24 µM. S130 suppresses autophagy flux[1]. | ||||||||||||
| Invitro | S130 suppresses autophagy and activates apoptosis by inhibiting ATG4B, leads to enhanced cytotoxicity[1].S130 (10 μM; 6 hours) suppresses autophagy at the early LC3 priming step or late autolysosome degradation stage[1].S130 accumulates autolysosomes with more lipidated LC3[1].S130 (0-25 μM; 48 hours) induces cell death through inhibiting the activity of ATG4B at a dose higher than 6.3 µM. And such cytotoxicity might not cause cell death through necroptosis[1].Nutrient deprivation enhances S130-induced cytotoxicity[1].S130 (0-10μM; 24 hours) suppresses approximately 79% of the cleavage of full-length LC3-GST at the 10 µM, while no substrates were processed in ATG4B KO cells. S130 displays obvious inhibitory effects on ATG4B[1]. Cell Cytotoxicity Assay[1] Cell Line: | ||||||||||||
| Name | S130 | ||||||||||||
| CAS | 1160852-22-1 | ||||||||||||
| Formula | C24H25N3O2 | ||||||||||||
| Molar Mass | 387.47 | ||||||||||||
| Transport | Room temperature in continental US; may vary elsewhere. | ||||||||||||
| Storage |
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