Robotnikinin_product_DataBase

Bioactivity	Robotnikinin is a small molecule capable of binding to and inhibiting the activity of Sonic Hedgehog (Shh) signaling up stream of Smo[1][2].
Invitro	Robotnikinin demonstrates ShhN-binding capacity at concentrations between 1.56 μM and 25 μM, with a KD of 3.1 μM derived from kinetic data[2].Robotnikinin (50 µM) prevents the ShhN-induced LC3-II increase[3].Robotnikinin (5 μM) downregulates classical NF-κB pathway proteins in H929 and U266 cell lines cocultured with HS-5 cells, suggesting a link between Hh signaling and the NF-κB pathway in MM[4]. RT-PCR[4] Cell Line:
Name	Robotnikinin
CAS	1132653-79-2
Formula	C25H27ClN2O4
Molar Mass	454.95
Transport	Room temperature in continental US; may vary elsewhere.
Storage	Please store the product under the recommended conditions in the Certificate of Analysis.
Reference	[1]. Manuel Hitzenberger, et al. The Binding Mode of the Sonic Hedgehog Inhibitor Robotnikinin, a Combined Docking and QM/MM MD Study. Front Chem [2]. Benjamin Z Stanton, et al. A small molecule that binds Hedgehog and blocks its signaling in human cells. Nat Chem Biol. 2009 Mar;5(3):154-6. [3]. Ronald S Petralia, et al. Sonic hedgehog promotes autophagy in hippocampal neurons. Biol Open. 2013 Apr 8;2(5):499-504. [4]. Ke Cai, et al. Targeting the cross-talk between the hedgehog and NF-κB signaling pathways in multiple myeloma. Leuk Lymphoma. 2019 Mar;60(3):772-781.

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